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以鲁豆１号作感病对照，通过接种相同数量的卵和二龄幼虫并采用切顶水培技术研究我国筛选出的两个小黑豆抗源：灰皮支黑豆和元钵黑豆对大豆孢囊线虫４号生理小种侵染和发育的影响。结果表明，接种后２４ｈ侵入大豆抗感品种根内的二龄幼虫数十分接近，差异不显著；接种后水培１５～１７ｄ，抗病品种根内的二、三龄幼虫数无论是单株平均值，还是所占百分数均极显著高于感病对照，而四龄幼虫、雌成虫和总成虫数正好与此相反。感病对照鲁豆１号根上二龄、三龄、四龄、雌成虫和总成虫数所占百分数为４．４０％、１０．２０％、２４．９５％、２９．３５％和６０．４５％，而元钵黑豆和灰皮支黑豆根上各龄线虫的百分数分别为２２．３０％、２６．５０％、１３．５５％、３．８０％、３７．６５％和２４．３０％、２９．６０％、１５．０５％、２．５０％、３１．１５％。抗感品种根上线虫性比的差异极明显，抗病品种为１０左右，而感病对照稍大于１；抗病品种根上线虫幼虫从二龄到三龄及从三龄到四龄阶段有较高的死亡率，而且从三龄到四龄阶段的死亡率高于从二龄到三龄阶段。
Taking Luodou No.1 as susceptible control, the two small black beans screened from China were screened by inoculation of the same number of eggs and second instar larvae, Effects of Cyst Nematode 4 Physiological Races on Infection and Development. The results showed that the number of second-instar larvae invaded into the root of susceptible soybean was nearly the same at 24 h after inoculation, and the difference was not significant. After inoculation, the number of second and third instar larvae Average, or the percentage were significantly higher than the susceptible control, and fourth instar larvae, adult female and the total number of adults exactly the opposite. The percentage of susceptible control Ludou No.1 rooted at the second, third and fourth instar, female adults and the total number of adults was 4.40%, 10.20%, 24.95%, 29.35% and 60.45 %, While the percentages of nematodes at each age of black bean and gray pea root in Yuanpao were 22.30%, 26.50%, 13.55%, 3.80%, 37.65% and 24.30%, respectively .60%, 15.05%, 2.50%, 31.15%. The difference of root nymphs in resistant varieties was very obvious. The resistant varieties were about 10, while the susceptible ones were slightly larger than 1. The root-knot nematode larvae of resistant cultivars were higher from the second to the third and from the third to the fourth Mortality rates from the third to the fourth age are higher than those from the second to the third age.