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一、人上皮细胞无血清培养系统的建立绝大多数人类恶性肿瘤起源于上皮细胞。上皮细胞的无血清培养技术的发展，对研究上皮细胞癌变及其机理具有重要意义。从1986年起，我们实验室先后建立了多种上皮细胞无血清培养系统。利用MCDB151加多种生长因子的无血清培养基，已成功培养了
First, the establishment of a serum-free culture system for human epithelial cells. The vast majority of human malignancies originate from epithelial cells. The development of serum-free culture technology of epithelial cells is of great significance for the study of epithelial canceration and its mechanism. Since 1986, our lab has established a variety of epithelial cell-free culture systems. Serum-free medium with MCDB151 plus various growth factors has been successfully cultivated