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目的 通过纯视细胞移植 ,观察重建视网膜神经传导通路中抑制性神经递质 γ-氨基丁酸(γ- Aminobutyric Acid,GABA)的分布和变化。 方法 取同龄 Wistar/皇家外科学院鼠 (royal college ofsurgeon rat,RCS)为供 /受体 ,准分子激光切削法制备视细胞层 ,外路途径移入 RCS鼠的视网膜下腔 ,术后 2周取 RCS鼠手术眼 ,手术对照眼 ,RCS鼠对照眼 ,Wistar鼠眼。分别做冰冻切片 ,免疫组织化学染色法染色 ,普通光学显微镜下观察。测量内丛状层 (inner plexiform layer,IPL )和节细胞层 (ganglion cell layer,GCL )的光密度 ,计数无长突细胞的数量 ,方差分析组间差异 ,配对 t检验 RCS鼠手术眼组内差异。 结果 在视细胞移植术后 2周 ,移植视细胞存活 ,外丛状层重建 ,与手术对照眼 ,RCS对照眼相比 ,受体 RCS鼠重建视网膜中内丛状层 GABA免疫反应阳性的神经纤维光密度测量值差异有显著性的意义 P<0 .0 0 1,而与 Wistar鼠眼比较无明显区别。和手术对照眼 ,RCS对照眼 ,Wistar鼠组比 ,GABA能无长突细胞 (amacrine cell)明显增多 (P<0 .0 5 )。节细胞层 GABA阳性免疫反应光密度测量值和上述几组比变化不明显 (P>0 .0 5 )。接受移植的 RCS鼠各部位的无长突细胞在移植部二侧和对侧赤道 ,后极部之间的差异无显著性的意义 (P
Objective To observe the distribution and changes of γ-aminobutyric acid (GABA), an inhibitory neurotransmitter in retinal nerve conduction pathway through pure-cell transplantation. Methods The retinoblastoma was prepared by excimer laser cutting with the same age Wistar / royal college of rat (rat) as the donor / recipient, and the extracellular route was transferred into the subretinal space of RCS rats. RCS Mouse eye surgery, surgery eye control, RCS rat eye control, Wistar rat eye. Frozen sections were made, stained with immunohistochemical staining, observed under ordinary light microscope. The optical densities of the inner plexiform layer (IPL) and the ganglion cell layer (GCL) were measured, the number of amacrine cells was counted, the differences among the groups were analyzed, and the paired t test was used to analyze the intraocular pressure difference. RESULTS: Two weeks after optic cell transplantation, the viable cells were transplanted and the outer plexiform layer was reconstructed. Compared with the control and RCS control eyes, recipient RCS mice reconstructed the GABA-immunoreactive nerve fibers in the retina There was a significant difference in optical density measurements P <0. 01, but no significant difference compared with Wistar rats. Compared with the control eyes, RCS control eyes and Wistar rats, the number of GABA amacrine cells increased significantly (P <0.05). The cell layer GABA-positive immune response optical density measurements and the above-mentioned several groups did not change significantly (P> 0.05). There was no significant difference between the two sides of the graft and the contralateral equator and posterior pole in allografts of the recipient RCS mice (P