采用薄层垂直平板聚丙烯酰胺凝胶电泳及光密度计扫描方法,对玉米一组同核异质系和一组同质异核系及相应保持系的花药(单核—双核期)进行了过氧化物酶、酯酶同工酶的比较分析。结果表明,两种酶的同工酶在同核异质系间和同质异核系间及与相应保持系间均表现不同程度的差异,两者可能由质核基因共同编码,但过氧化物酶同工酶是胞质基因占主导,酯酶同工酶是核基因占主导。本文并就细胞质雄性不育的机制进行了讨论。 A set of anther nuclear (mononuclear - dinuclear) populations from a set of isonuclear alloplasmic and homologous heterokaryotic lines and corresponding maintainer lines of maize were studied by thin-layer vertical plate polyacrylamide gel electrophoresis and densitometry scanning Peroxidase, esterase isozymes comparative analysis. The results showed that the isoenzymes of the two isoenzymes all showed different degrees of differentiation between the iso-heterologous lines and the homozygous lines and their maintainer lines, both of which may be co-encoded by the mitochondrial genes, but the peroxidase Enzymes isoenzyme is the dominant cytoplasmic gene, esterase isozyme is the dominant nuclear gene. This paper also discussed the mechanism of cytoplasmic male sterility.