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合成功能单体N,O-双异丁烯酰乙醇胺(N,O-bismethacryloyl ethanolamine,NOBE),采用表面分子印迹技术,以硅胶为载体,乙二醇二甲基丙烯酸酯(ethylene glycol dimethacrylate,EGDMA)为交联剂,制备乙氧酰胺苯甲酯(ethopabate,ETP)分子印迹聚合物(molecularly imprinted polymers,MIPs)。采用正交试验方法,对ETPNOBE物质的量比、ETP-EGDMA物质的量比和致孔剂种类3个因素进行考察,结果表明:3个影响因素主次顺序为致孔剂种类>ETP-NOBE物质的量比>ETP-EGDMA物质的量比;当致孔剂为乙腈、ETP-NOBE-EGDMA的物质的量比为1:2:20时,所制备的ETP-MIPs印迹效果最佳。Scatchard模型研究发现存在两类结合位点,高亲和位点与低亲和位点的平衡离解常数K_d和最大表观结合量Q_(max)分别为K_(d1)=1.608μg/mL,Q_(max1)=1.101μg/mg;K_(d2)=0.109μg/mL,Q_(max2)=0.172μg/mg。
Synthesis of N, O-bismethacryloyl ethanolamine (N, O-bismethacryloyl ethanolamine, NOBE) was investigated by surface molecular imprinting technique and silica gel as carrier. Ethylene glycol dimethacrylate (EGDMA) Crosslinkers were prepared for ethlyphedrin (ETP) molecularly imprinted polymers (MIPs). Orthogonal test method was used to investigate the ratio of ETPNOBE, the ratio of ETP-EGDMA and the type of porogen. The results showed that the order of the three influencing factors was ETO-NOBE The amount ratio of ETP-EGDMA was the ratio of ETP-EGDMA. When the ratio of ETP-NOBE-EGDMA was 1: 2: 20, the best ETP-MIPs was obtained when the porogen was acetonitrile. The Scatchard model shows that there are two types of binding sites, the equilibrium dissociation constant K_d and the maximum apparent binding capacity Q_ (max) of high affinity and low affinity sites are K_ (d1) = 1.608μg / mL, Q_ (max1) = 1.101 μg / mg; K d2 = 0.109 μg / mL and Q max2 = 0.172 μg / mg.