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目的建立高效液相色谱法测定骨痹散中延胡索乙素含量的方法。方法 DIONEX-C18色谱柱(250mm×4.6mm,5μm),乙腈-0.1%磷酸溶液(25:75)为流动相,柱温为30℃,流速为1.0m L/min,进样量10μL,检测波长为300nm。结果延胡索乙素在10.4208-74.1385μg表现良好线性关系(r=0.9997),平均加样回收率为99.1143%,RSD=0.6623%。结论本方法准确、简便、快速,且重复性好,可用于骨痹散中延胡索乙素含量测定及质量控制。
Objective To establish a method for the determination of tetrahydropalmatine in Gubisan by high performance liquid chromatography. Methods The mobile phase consisted of DIONEX-C18 column (250 mm × 4.6 mm, 5 μm) and acetonitrile-0.1% phosphoric acid solution (25:75) with a column temperature of 30 ℃ and a flow rate of 1.0 mL / The wavelength is 300 nm. Results Tetrahydropalmatine showed a good linearity (r = 0.9997) at 10.4208-74.1385μg with an average recovery of 99.1143% and a RSD of 0.6623%. Conclusion The method is accurate, simple, rapid and reproducible. It can be used for the determination and quality control of tetrahydropalmatine in Gubi San.