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为研究儿茶素的抗氧化作用,以分光光度法测定了其对红细胞溶血和肝线粒体肿胀的保护作用;用TBARA法测定了其对血清自氧化、肝匀浆自发性及Fe2+诱导性脂质过氧化的抑制作用;并测定了其对·OH、O2-·以及DPPH自由基清除能力和对铁离子的还原能力。结果表明:儿茶素对Fe3+的还原能力是Vc的11.76%;能有效清除Fenton反应生成的·OH和次黄嘌呤-黄嘌岭氧化酶系统产生的O2-·以及DPPH自由基,其IC50分别为89.62、29.79μmol/L和6.59μmol/L;对红细胞溶血和血清自氧化均具有很好的保护作用,并能有效地抑制肝匀浆自发性和Fe2+诱导性脂质过氧化。这些结果说明儿茶素具有明显的抗氧化活性。
In order to study the antioxidant effect of catechin, its protective effect on hemolysis of erythrocytes and swelling of liver mitochondria was determined by spectrophotometry. The effect of catechin on serum autoxidation, spontaneous hepatic homogenate and Fe2 + -induced lipid Peroxidation, and its scavenging ability on · OH, O2- · and DPPH free radicals as well as reducing ability to iron ions were determined. The results showed that the reducibility of catechin to Fe3 + was 11.76% of that of Vc; the scavenging ability of · OH and · OH and hypoxanthine-xanthine oxidase produced by Fenton reaction and DPPH free radical were effectively eliminated with IC50 of 89.62 , 29.79μmol / L and 6.59μmol / L respectively. It has good protective effect on erythrocyte hemolysis and serum autoxidation, and can effectively inhibit hepatic homogenate spontaneous and Fe2 + -induced lipid peroxidation. These results show that catechins have obvious antioxidant activity.