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利用组织培养方法研究艾西丝南瓜适于生产上应用的种苗快繁技术。试验结果表明:艾西丝南瓜分化培 养基以 MS + BA10 mg/L + IAA01 ~05m g/L 最好;壮苗培养以 MS+ BA05m g/L+ PP33301 ~05 mg/L 为宜;适宜于试管苗生根的培养基为1/2 MS0 ;适宜的试管苗移栽驯化基质为细沙、腐叶土、粗沙分层基质。用组培手段培育的试管苗种性不变异,生长、结果习性优于种子苗
Tissue culture method was used to study the rapid propagation of seedlings of Aisi pumpkin suitable for production. The results showed that: MS medium BA1.0 mg / L + IAA0-1.5 mM / L was the best medium for the culture of Asiatic pumpkin; MS + BA0.5 mg / L + PP3330-1-0 5 mg / L is appropriate; suitable medium for rooting of test-tube seedlings is 1/2 MS0; the suitable medium for transplanting and acclimatization of test-tube seedlings is fine-grained, rot-leaf soil and coarse-grained layered substrate. Histology of tissue culture with tissue culture means no variation, growth, the results of habitat better than seedlings