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旨在建立一种分泌型荧光素酶基因标记的小鼠原位移植型肝癌模型并观察其对干扰素β基因治疗的反应。首先建立稳定表达分泌型荧光素酶Gluc(Gaussia princeps luciferase)的小鼠肝癌细胞Hepa 1-6/Gluc;将该细胞通过脾注射至C57BL/6小鼠肝脏建立原位移植型肝癌模型,通过检测外周血Gluc活性监测小鼠体内肿瘤生长情况;用此模型观察水动力注射干扰素β质粒DNA的抗肿瘤效果。结果表明,通过脾注射Gluc基因标记的Hepa 1-6细胞可以建立小鼠原位移植型肝癌模型;外周血Gluc活性可以有效反映体内接种肿瘤细胞的数量和肿瘤的生长情况;通过监测外周血Gluc活性可灵敏反映干扰素β基因治疗对肿瘤生长的抑制作用。本研究表明,利用Gluc为报告基因建立的小鼠原位移植型肝癌模型可以体外实时监测肿瘤的生长情况,并能灵敏可靠地用于抗肿瘤治疗效果的评价。
Aim To establish a murine orthotopic liver transplantation model labeled with secreted luciferase gene and observe its response to interferon β gene therapy. Firstly, Hepa 1-6 / Gluc, a mouse hepatoma cell stably expressing the Gaussia princeps luciferase, was established. The cells were injected into the liver of C57BL / 6 mice via spleen to establish a model of orthotopic liver transplantation. Peripheral blood Gluc activity was used to monitor tumor growth in mice. The model was used to observe the anti-tumor effects of hydrodynamic injection of interferon beta plasmid DNA. The results showed that mouse model of orthotopic liver transplantation can be established by injecting Gluc gene-labeled Hepa 1-6 into the spleen. Peripheral blood Gluc activity can effectively reflect the number of inoculated tumor cells and the growth of tumor. By monitoring Gluc Activity can be sensitive to reflect the interferon β gene therapy on tumor growth inhibition. This study showed that the use of Gluc reporter gene established mouse orthotopic liver transplantation model in vitro monitoring of tumor growth in real time and can be sensitive and reliable for the evaluation of anti-tumor effect.