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利用微型表面等离子体共振的生物传感器测量了残留在牛奶中的氨苄青霉素的浓度.采用竞争抑制试验的方法,即先将定量的单克隆抗氨苄青霉素抗体(3H295)和含氨苄青霉素的牛奶样品混合,样品中氨苄青霉素即与抗体结合,然后将该混合样品通入传感器表面,传感器的表面共价固定了氨苄青霉素分子,通过生物特异相互作用分析,检测样品中剩余的抗体,从而得到氨苄青霉素的浓度.样品的测量时间为10 m in,最低检测限为2.5 ng/mL,低于欧盟标准4 ng/mL.该检测方法的测量时间短、重复性好,批间测量的变异系数为5.4%,表明该方法能满足实际测量要求.
The concentration of ampicillin remaining in the milk was measured using a micro-surface plasmon resonance biosensor using a competitive inhibition assay by first combining a quantitative monoclonal anti-ampicillin antibody (3H295) with an ampicillin-containing milk sample , Ampicillin is bound to the antibody in the sample, and the mixed sample is passed to the sensor surface. The ampicillin molecule is covalently immobilized on the surface of the sensor. The remaining antibody in the sample is detected by means of a biological interaction analysis to obtain ampicillin Concentration.The sample measurement time is 10 min, the minimum detection limit is 2.5 ng / mL, lower than the EU standard 4 ng / mL.The detection method of the measurement time is short, good repeatability, inter-batch measurement coefficient of variation of 5.4% , Indicating that the method can meet the actual measurement requirements.