论文部分内容阅读
[目的]研究生人工老化对向日葵种子活力及生理特性的影响。[方法]以‘Sunm20’、‘民乐深紫葵’、‘大山葵花4’和‘吉葵24’4份向日葵种质为材料,研究了高温高湿人工老化(100%RH,45℃)处理不同时间(0、2、4、6、8、10d)对种子发芽率、发芽势、发芽指数、活力指数、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响。[结果]随着向日葵种子老化时间的延长,发芽率、发芽势、发芽指数、活力指数、POD和SOD活性逐渐下降,MDA含量逐渐增加;不同种质的抗老化能力存在差异,其中‘Sunm20’比其他3份种质的抗老化能力强,在相同条件下,其种子活力和保护酶活性最高,MDA含量最低,并且在老化过程中,其种子活力变化及保护酶活性变化最慢。[结论]老化过程中保护酶POD、SOD活性的显著降低可能是导致种子活力下降的主要原因,而少量MDA的逐渐积累促进了种子的老化。
[Objective] The research aimed to study the effects of artificial aging on the vigor and physiological characteristics of sunflower seeds. [Method] The germplasms of Sunflower (Sunm20 ’),’ Mandelia spp. ’,’ Sunflower 4 ’and’ 24’4 parts of sunflower were used as materials to study the effects of artificial aging under high temperature and humidity (100% RH, 45 ℃) (0, 2, 4, 6, 8, 10d) on seed germination rate, germination potential, germination index, vigor index, peroxidase (POD), superoxide dismutase (SOD) activity and malondialdehyde MDA) content. [Result] The germination rate, germination potential, germination index, vigor index, POD and SOD activity decreased gradually and the content of MDA increased gradually with the aging time of sunflower seed. The anti-aging ability of ’Sunm20’ Compared with the other three germplasms, the anti-aging ability was the highest. Under the same conditions, the seed vigor and the protective enzyme activities were the highest, and the MDA content was the lowest. And the changes of the seed vigor and the protective enzyme activities were the slowest during the aging process. [Conclusion] The significant decrease of POD and SOD activity during the aging process may be the main reason for the decrease of seed vigor. However, the gradual accumulation of a small amount of MDA promoted the seed aging.