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采用花粉管通道途径，将合成的Ｂｔ杀虫蛋白基因导入棉花优良品种泗棉３号和中棉所１２，获得了转基因植株。组织化学分析表明，ＧＵＳ标记基因已在Ｒ１代植株中表达。根据对ＧＵＳ阳性转基因植株的ＰＣＲ分析，证明Ｂｔ杀虫蛋白基因出现在Ｒ１代植株中，并通过自交传递至Ｒ２代。经抗虫性鉴定，获得了５株对棉铃虫有高度毒杀作用的Ｒ１代转基因抗虫植株Ｓ５４５、Ｓ５９１、Ｓ６３６、Ｓ１００１（泗棉３号＋Ｂｔ／ＧＵＳ）和Ｚｈ１１０９（中棉所１２＋Ｂｔ／ＧＵＳ），对棉铃虫幼虫的致死率分别达到９１．６％、９３．８％、９２．３％、８５．７％和７５．０％。通过自交和选育，获得了Ｒ５代转基因抗虫棉，并进行了抗虫性和转基因的跟踪鉴定，表明通过基因工程方法获得了抗棉铃虫的棉花新种质。
The pollen tube pathway was used to transfer the synthesized Bt insecticidal protein gene into cotton elite variety Simian 3 and Cummins 12, and transgenic plants were obtained. Histochemical analysis showed that the GUS marker gene was expressed in R1 plants. Based on PCR analysis of GUS-positive transgenic plants, it was demonstrated that the Bt insecticidal protein gene was present in the R1 plants and was selfed to the R2 generation. Five insect-resistant plants R1, S545, S591, S636, S1001 (Simian 3 + Bt / GUS) and Zh1109 (CIMC 12 + Bt / GUS) ), The lethal rates of H. armigera larvae reached 91.6%, 93.8%, 92.3%, 85.7% and 75.0% respectively. Through selfing and breeding, the R5 transgenic cotton was obtained, and the insect resistance and transgene tracing identification were carried out. The results showed that new cotton germplasms resistant to cotton bollworm were obtained through genetic engineering.