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利用自由基聚合反应将甲基丙烯酸聚乙二醇酯(PEGMA)和甲基丙烯酸缩水甘油酯(GMA)的二元共聚物接枝在基材表面,并通过开环反应分别固定精氨酸-甘氨酸-天冬氨酸(RGD)、精氨酸-谷氨酸-天冬氨酸-缬氨酸(REDV)和酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸(YIGSR)3种可特异性黏附内皮细胞的多肽.通过核磁共振检测合成的聚合物分子结构,并进一步通过X射线光电子能谱(XPS))以及原子力显微镜(AFM)的测试结果证明聚合物成功接枝在基材表面.利用紫外-可见吸收光谱(UV-Vis)对表面固定的3种多肽进行了定量表征.体外内皮细胞和平滑肌细胞黏附结果表明,3种不同多肽修饰的共聚物表面均能够有效阻抗平滑肌细胞的黏附,同时不同程度地促进内皮细胞的黏附,从而实现了基材表面内皮细胞的选择性黏附.其中与RGD和YIGSR多肽修饰的表面相比,REDV多肽修饰的表面呈现出更优异的内皮细胞选择性.这种具有内皮细胞特异选择性的界面在心血管支架涂层原位内皮化方面具有良好的应用前景.
The binary copolymer of polyethylene glycol methacrylate (PEGMA) and glycidyl methacrylate (GMA) was grafted onto the surface of the substrate by free radical polymerization and the arginine- Glycine-Aspartic acid (RGD), Arg-Glu-Asp (REDV) and Tyrosine-Isoleucine-Glycine-Serine- Arginine Endothelial cells can be specifically adhered to the polypeptide through the detection of the molecular structure of the polymer synthesized by nuclear magnetic resonance and further by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) test results show that the successful grafting of polymers in the base The three surface-immobilized polypeptides were quantitatively characterized by ultraviolet-visible absorption spectroscopy (UV-Vis) .The results of in vitro adhesion of endothelial cells and smooth muscle cells showed that the copolymer surface modified by three different peptides could effectively inhibit the smooth muscle Cell adhesion, and to some extent promote the adhesion of endothelial cells, so as to achieve the selective adhesion of endothelial cells on the substrate surface.Which compared with RGD and YIGSR polypeptide modified surface, REDV polypeptide modified surface showed a more excellent endothelial fine Cell-selective. This endothelial-specific interface has good potential for in situ endothelialization of cardiovascular scaffolds.