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[目的]构建线粒体LETM1基因真核表达载体.[方法]从肾癌细胞株293T细胞中提取mRNA及合成cDNA,设计并合成线粒体LETM1基因引物,用PCR方法扩增LETM1基因,连接到真核表达载体pCMV-3Tag-6上,构建重组质粒pCMV-3Tag-LETM1,经酶切和测定序列进行鉴定.[结果]构建了重组质粒pCMV-3Tag-LETM1.[结论]成功地构建了线粒体LETM1真核表达载体.
[Objective] To construct the eukaryotic expression vector of mitochondrial LETM1 gene. [Methods] mRNA was extracted from 293T cells and cDNA was synthesized. The mitochondrial LETM1 gene was designed and synthesized. The LETM1 gene was amplified by PCR and ligated into eukaryotic expression vector [Results] The recombinant plasmid pCMV-3Tag-LETM1 was constructed. [Conclusion] The recombinant plasmid pCMV-3Tag-LETM1 was successfully constructed. Expression vector.