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目的建立大鼠血浆中阿霉素浓度的液相色谱-串联质谱(LC-MS/MS)测定方法。方法色谱柱:Venusil ASB C18(150 mm×4.6 mm,5μm),流动相:乙腈-5 mmol.L-1乙酸铵-甲酸(体积比为35.0∶65.0∶0.5),采用沉淀蛋白法,以多反应监测(multiple reaction monitoring,MRM)扫描方式检测,测定大鼠尾静脉注射阿霉素纳米胶束溶液后血浆中药物浓度。结果血浆中阿霉素质量浓度在1～1 000μg.L-1内线性关系良好,r=0.996 0;日内和日间精密度RSD≤13.2%;阿霉素的平均提取回收率为89.7%～95.7%;阿霉素在大鼠血浆中主要药动学参数为t1/2(30.5±5.2)h,ρmax(954.3±80.6)μg.L-1,AUC0-∞/(290.2±40.4)μg.h.L-1。结论该方法适用于阿霉素在大鼠体内药动学的研究。
Objective To establish a method for the determination of doxorubicin in rat plasma by liquid chromatography-tandem mass spectrometry (LC-MS / MS). Methods The column was Venusil ASB C18 (150 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-5 mmol·L -1 ammonium acetate-formic acid (volume ratio 35.0:65.0:0.5) Multiple reaction monitoring (MRM) was used to detect the plasma concentrations of doxorubicin in rat tail vein. Results The linearity of doxorubicin in plasma was linear in the range of 1-1000 μg · L-1, r = 0.996 0; the RSD of intra-day and inter-day was ≤13.2%; the average recovery of adriamycin was 89.7% 95.7% respectively. The main pharmacokinetic parameters of doxorubicin in rat plasma were t1 / 2 (30.5 ± 5.2) h, ρmax (954.3 ± 80.6) μg.L-1, and AUC0-∞ / (290.2 ± 40.4) μg. hL-1. Conclusion This method is suitable for the study of pharmacokinetics of doxorubicin in rats.