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采用组织病理学和酶组织化学方法对比了沙鼠胆脂瘤造模和人的胆脂瘤的特征,阐明胆脂瘤发生及发展的机制。标本采集:4～6月龄蒙古沙鼠(8只),腹腔内麻醉,无菌条件下,靠近听泡处细丝线缝扎两侧外耳道,造成胆脂瘤。8个月活杀动物取胆脂瘤标本。手术切除病人胆脂瘤标本(共13名病人,女8、男5,年龄14～77岁,都有慢性耳疾和胆脂瘤)及周围上皮、耳道皮肤和鼓膜。并取健康耳道皮肤(耳硬化症手术中)作为对照。光镜观察和酶组织化学方法:所取沙鼠和人的胆脂瘤标本经甲醛固定、脱钙、石蜡包埋,5μm厚切片,苏木素、伊红染色。另一沙鼠标本不用
Histopathological and enzymatic histochemical methods were used to compare the characteristics of gerbil cholesteatoma model and human cholesteatoma and elucidate the mechanism of the occurrence and development of cholesteatoma. Specimens were collected: Mongolian gerbils aged from 4 to 6 months (n = 8) were anesthetized intraperitoneally and sutured to the external auditory meatus on the both sides of the fine thread at the ear canal, under aseptic conditions, resulting in cholesteatoma. 8 months to kill animals take cholesteatoma specimens. Surgical resection of patients with cholesteatoma specimens (a total of 13 patients, 8 females, 5 men, aged 14 to 77 years, have chronic ear diseases and cholesteatoma) and the surrounding epithelium, ear canal skin and tympanic membrane. Take healthy ear canal skin (otosclerosis surgery) as a control. Light microscopy and enzymatic histochemistry: Gerbils and human cholesteatoma specimens were fixed with formaldehyde, decalcified, embedded in paraffin, sliced at 5 μm, stained with hematoxylin and eosin. No other gerbil specimens