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目的:研究食管癌细胞的分化状态对ALA产生的内源性光敏剂PpⅨ累积水平的影响,及对PDT的应答敏感程度。方法:以高分化食管鳞癌KYSE-450细胞和低分化食管鳞癌KYSE-70细胞为研究对象,利用荧光分光光度法测定不同浓度ALA处理后细胞内PpⅨ的浓度;分别使用不同剂量的红光和蓝光对细胞
OBJECTIVE: To study the effect of esophageal cancer cell differentiation status on the accumulation level of endogenous photosensitizer PpⅨ produced by ALA and the sensitivity to PDT. Methods: KYSE-450 cells and poorly differentiated esophageal squamous cell carcinoma KYSE-70 cells were divided into two groups. The concentrations of PpⅨ in cells treated with different concentrations of ALA were measured by fluorescence spectrophotometry. Different doses of red light And blue light on the cells