黑热病仍是印度东部省区的重要卫生问题之一。随时都可用某些血清学方法对此病作特异和敏感的诊断。但是学者们仍在持续寻找对此病简易、特异、敏感和经济的血清诊断方法。本文报告用杜氏利什曼原虫前鞭毛体于显微镜下观察的凝集试验的实验结果。 将杜氏利什曼原虫置于改良的Ray’s培养基中(Majumder等,1990),在25℃下培 Kala-azar is still one of the major health problems in the eastern provinces of India. Certain serological methods may be used to make specific and sensitive diagnoses of the disease at any time. However, scholars are still searching for simple, specific, sensitive and economical serodiagnostic methods for the disease. This article reports the experimental results of an agglutination test observed with Leishmania donovani promastigotes microscopically. Leishmania donovani was placed in a modified Ray’s medium (Majumder et al., 1990), cultured at 25 ° C