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目的了解鼠类携带肾综合征出血热(HFRS)病毒情况,为制订防制措施提供科学依据。方法采用人工挖、放鼠笼及夹夜法等方式捕获鼠类,无菌解剖取肺,编号,冰冻切片,冷丙酮固定,进行LFT染色,用免疫荧光技术检测HFRS病毒抗原。结果捕获鼠类301只,5个品种,黑线姬鼠占捕获总数的44.19%,为优势种。在301只鼠类中,有43只鼠检出HFRS病毒抗原,带毒率为14.29%。5种鼠有4种携带HFRS病毒抗原,鼠类间带毒率比较经统计学处理,差异无统计学意义(χ~2=3.49,P>0.05)。结论大竹县鼠类携带HFRS病毒率高,需采取有效的综合防制措施,控制鼠类密度,从而有效控制HFRS的发生和流行。
Objective To understand the situation of murine hemorrhagic fever with renal syndrome (HFRS) virus and provide a scientific basis for the development of control measures. Methods The mice were harvested by artificial digging, squirrel cage and nocturnal nocturnal extraction, and the lungs were dissected by aseptic dissection, numbered, frozen and fixed with cold acetone for LFT staining. The antigens of HFRS virus were detected by immunofluorescence technique. Results 301 cats and 5 species of rodents were captured, accounting for 44.19% of the total number captured, which was the dominant species. Of 301 mice, 43 mice detected HFRS virus antigens with a rate of 14.29%. There were 4 kinds of 5 kinds of mice carrying HFRS virus antigens, and there was no significant difference in the virulence rate between mice (χ ~ 2 = 3.49, P> 0.05). Conclusion The incidence of HFRS in rodents in Dazhu County is high, and effective control measures should be taken to control the density of rodents so as to effectively control the occurrence and prevalence of HFRS.