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目的建立咽炎合剂的质量标准。方法采用薄层色谱(TLC)对咽炎合剂中金银花、玄参、甘草进行定性鉴别,以高效液相色谱法(HPLC)对主药金银花中绿原酸进行含量测定。色谱柱为Wondasi C18柱,流动相为甲醇-水-冰醋酸(20:80:1),检测波长为327nm;柱温为室温,流速为1.0ml/min。结果咽炎合剂中金银花、玄参、甘草的TLC中,在与对照品色谱相应的位置上,显相同颜色的斑点;绿原酸在0.074～1.184μg间有良好的线性关系(r=0.9999)平均回收率为99.30%,RSD为1.17%。结论本方法简便、快速、准确、灵敏、重复性好,可作为咽炎合剂的质量控制。
Objective To establish the quality standard of pharyngitis mixture. Methods Thin-layer chromatography (TLC) was used to qualitatively identify honeysuckle, Scrophularia and Glycyrrhiza in Pharyngeal Mixture. The content of chlorogenic acid in honeysuckle was determined by high performance liquid chromatography (HPLC). The column was a Wondasi C18 column, and the mobile phase was methanol-water-glacial acetic acid (20:80:1). The detection wavelength was 327 nm. The column temperature was room temperature and the flow rate was 1.0 ml/min. RESULTS: TLC of honeysuckle, Scrophularia and Glycyrrhizae in Pharyngitis mixture had the same color spots at the positions corresponding to the chromatograms of the reference substance; there was a good linear relationship (r=0.9999) between chlorogenic acid between 0.074 and 1.184 μg. The recovery rate was 99.30% and the RSD was 1.17%. Conclusion This method is simple, rapid, accurate, sensitive, and reproducible, and can be used as a quality control of pharyngitis mixture.