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Based on the specific primer from the nucleotide sequences between 16S-23S rDNA,Polymerase chain reaction(PCR) approach for detecting Leifsonia xyli subsp.xyli(Lxx) was established.The approach was applied to detect Lxx from 30 cultivars collected from two main sugar cane producing areas in Yunnan and 10 hot-water treatment samples.The results showed that 80% of the cultivars were infected by Lxx and hot-water treatment was proved to be an efficient measure to control but not completely eliminate Lxx in sugar cane tissues.The PCR products amplified from six infected cultivars were cloned and sequenced,six sequences were acquired and analyzed.It indicated that the six sequences were identical and showed 100% similarity with the isolates from Brazil,Australia and Fujian,and 99.54% with the isolate from Louisiana.
Based on the specific primer from the nucleotide sequence between 16S-23S rDNA, Polymerase chain reaction (PCR) approach for detecting Leifsonia xyli subsp.xyli (Lxx) was established. The approach was applied to detect Lxx from 30 cultivars collected from two main sugar cane producing areas in Yunnan and 10 hot-water treatment samples. The results showed that 80% of the cultivars were infected by Lxx and hot-water treatment was proved to be an efficient measure to control but not completely eliminate Lxx in sugar cane tissues. The PCR products amplified from six infected cultivars were cloned and sequenced, six sequences were acquired and analyzed. It indicates that the six sequences were identical and showed 100% similarity with the isolates from Brazil, Australia and Fujian, and 99.54% with the isolate from Louisiana.