微电极是生理学研究中常用的实验工具之一。记录单一肌纤维和神经元的电活动,必须要有制备精良的微电极才能完成,同时微电极绝缘程度的高低,直接关系着实验的成败。因此微电极涂漆(或其他绝缘物)是制作微电极过程中的重要步骤。传统的涂漆方法是,将电极徐徐插入漆内,稍加停留,缓缓上提,然后将电极翻转静置,最后烘烤。这种涂漆方法费时多,效果差,有时还需提插装置。我们针对这些问题作了相应的改进,摸索了一套快速,简便,效果较好的涂漆方法,简介如下: 一涂漆棉花框的制作先将棉花纤维整理成束,然后加以对折,厚度约2毫米。另用漆皮线(φ1毫米)折成形框架两个(15毫米×15毫米)再将棉花束夹在中间,并用文具夹夹住棉花框,放在合适的台架上,临用前将棉花框浸漆。见图1: Microelectrode is one of the commonly used experimental tools in physiological research. To record the electrical activity of a single muscle fiber and neurons, we must have a well-prepared microelectrode to complete, while the level of microelectrode insulation, directly related to the success of the experiment. Microelectrode painting (or other insulation) is therefore an important step in the fabrication of microelectrodes. The traditional method is to paint the electrode slowly into the paint, a little stay, slowly lift, and then flip the electrode stand, and finally baked. This lacquering method is time-consuming, poor results, and sometimes need to mention plug device. We have made corresponding improvements on these issues, and explored a set of quick, easy and effective paint methods. The brief introduction is as follows: The production of a painted cotton box first arranges the cotton fibers into bundles, then folds them, the thickness is about 2 mm. Another use of patent leather line (φ1 mm) folded frame two (15 mm × 15 mm) and then the cotton bundle sandwiched between and clip the cotton box with stationery, on a suitable bench, before the cotton box Impregnation. see picture 1: