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目的:探讨和解利湿方通过抑制胰腺腺泡细胞凋亡对大鼠乙醇性慢性胰腺炎(ACP)的治疗作用。方法:将受试动物30只,随机分为2组,正常组6只,造模组24只,给予等热量的Lieber-De-Carli流质饮食,每日消毒后定量喂食14周;正常对照组6只,饲普通饮食14周;第8周末始模型组大鼠尾静脉注射给予脂多糖(LPS)剂量分别为1.5,1.5,2.0,2.5,3 mg.kg-1,1周1次,共5次,对照组给予等剂量的生理盐水;10周末造模组重分为模型组、和解利湿方高、中、低剂量(21.7,10.85,5.425 g.kg-1)治疗组,每组6只,开始ig给药,每天1次,连续给药4周。测定其组织病理变化、细胞凋亡及其相关蛋白的表达。体外观察含药血清对胰腺腺泡AR42J细胞生长和凋亡的影响。结果:与模型组比较,正常组无明显炎症症状,而和解利湿方组能减少炎症细胞浸润与胰腺腺泡细胞脱落,差异有统计学意义(P<0.05);和解利湿方高剂量组减少细胞凋亡,有显著的统计学差异(P<0.05),并能抑制半胱氨酸天冬氨酸蛋白酶(caspase)-3和caspase-9活性,增加B细胞淋巴细胞/白血病2基因(Bcl-2),降低Bcl-2相关蛋白基因(Bax)表达,其中和解利湿方高剂量组中Bcl-2/Bax是模型组的1.5倍。体外试验与含30%正常大鼠血清组比较,含30%和解利湿方药物血清明显促进胰腺腺泡细胞的生长(P<0.05);与乙醛干预并加正常大鼠血清组比较,和解利湿方含药血清组细胞凋亡率减少了32.66%。结论:高剂量的和解利湿方对ACP有一定的治疗作用,其机制可能与减少腺泡细胞凋亡、调节胰腺细胞caspase-3,caspase-9的活性及Bcl-2和Bax蛋白的表达有关。
OBJECTIVE: To investigate the therapeutic effect of Lixin Prescription on rat chronic alcohol-induced chronic pancreatitis (ACP) by inhibiting pancreatic acinar cell apoptosis. Methods: Thirty animals were randomly divided into two groups: normal group (n = 6) and model group (n = 24). The rats were given the same amount of heat as the Lieber-De-Carli liquid diet for 14 weeks after disinfection. 6 rats were fed with normal diet for 14 weeks. At the end of the 8th week, the mice in the model group were injected intravenously with LPS for 1.5, 1.5, 2.0, 2.5, 3 mg.kg-1, and 1 week respectively The rats in the control group were given the same dose of normal saline. The rats in the 10-week-old model group were divided into model group and high-dose and low-dose (21.7, 10.85, and 5.425 g.kg-1) 6, began to ig administration, 1 day, continuous administration for 4 weeks. The histopathological changes, apoptosis and the expression of related proteins were determined. Effect of Serum Contained in Vitro on the Growth and Apoptosis of Pancreatic Acinus AR42J Cells in. Results: Compared with the model group, there was no obvious inflammatory symptom in the normal group, while the Xieli Wet Decoction group could reduce inflammatory cell infiltration and pancreatic acinar cell shedding, the difference was statistically significant (P <0.05) (P <0.05), inhibited the activity of caspase-3 and caspase-9 and increased the expression of B lymphocyte / leukemia 2 gene ( Bcl-2, Bcl-2 related protein gene (Bax) expression, in which the Bcl-2 / Bax high-dose group and the solution of dampness prescription 1.5 times. Compared with 30% normal rat serum group, the serum containing 30% Heshitang decoction significantly promoted the growth of pancreatic acinar cells in vitro (P <0.05). Compared with the control group and the normal rat serum group, the reconciliation Lishi wet drug-containing serum apoptosis rate decreased by 32.66%. CONCLUSION: High-dose Jielu Lishi decoction has a certain therapeutic effect on ACP. The mechanism may be related to the reduction of acinar cell apoptosis, the activity of caspase-3 and caspase-9 in pancreatic cells and the expression of Bcl-2 and Bax proteins .