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目的:建立HPLC法测定不同产地半夏中鸟苷和尿苷的含量。方法:收集10个不同产地半夏药材,采用HPLC法测定半夏中有效成分鸟苷和尿苷含量,Hypersil ODS(5μm,4.6×150 mm)色谱柱,柱温:25℃,流动相:水∶甲醇=95∶5,检测波长254 nm,流速1 m L/min。结果:鸟苷在0.33μg/m L~26μg/m L与峰面积线性关系良好(r=0.999 7,P<0.000 1),尿苷在0.35μg/m L~28μg/m L与峰面积线性关系良好(r=0.999 9,P<0.000 1)。10种不同产地半夏中2种核苷含量之和大小为:甘肃清水>甘肃天水>甘肃西和>四川南充>重庆垫江>四川西昌>湖南邵东>贵州威宁>贵州水城>云南文山。结论:本法简便、准确、可靠,可作为半夏质量控制方法之一。半夏中的核苷含量从东南到西北逐渐增加,且不同居群半夏药材的鸟苷与尿苷含量都存在显著性差异,鸟苷与尿苷的含量总和分析为半夏药材的质量评价提供了依据。
Objective: To establish an HPLC method for the determination of guanosine and uridine in different regions of Pinellia ternata. Methods: Ten Chinese medicinal materials of Pinellia ternata were collected. The content of guanosine and uridine in the Pinellia ternata was determined by HPLC. The column of Hypersil ODS (5μm, 4.6 × 150 mm) was used. The column temperature was 25 ℃ and the mobile phase was water : Methanol = 95: 5, detection wavelength 254 nm, flow rate 1 m L / min. Results: The linear relationship between the concentration of guanosine and the peak area was good (r = 0.999 7, P <0.000 1) at 0.33μg / m L ~ 26μg / m L, Good relationship (r = 0.999 9, P <0.000 1). The sum of the content of two kinds of nucleosides in Pinellia ternata in 10 different habitats was as follows: Gansu Qingshui> Tianshui of Gansu> Xixi of Gansu> Nanchong of Sichuan> Dianjiang of Chongqing> Xichang of Sichuan> Shaodong of Hunan> Weining of Guizhou> Watertown of Guizhou> Wenshan of Yunnan. Conclusion: This method is simple, accurate and reliable and can be used as one of the quality control methods for Pinellia ternata. The content of nucleosides in Pinellia ternata gradually increased from southeast to northwest, and the contents of guanosine and uridine in different populations of Chinese Pinellia ternata were significantly different. The total content of guanosine and uridine was the quality evaluation Provided the basis.