12 9尾中国对虾 (Penaeuschinensis)分别捕自未暴发白斑综合症 (WSSV病毒所致 )虾池、WSSV暴发虾池以及曾暴发WSSV虾池。用斑点杂交和组织病理学方法确定各尾对虾的染毒 (WSSV)程度。用 96孔酶标板法测量相应个体血淋巴上清液的抗菌活力 (Ua)、溶菌活力 (UL)、酚氧化酶 (PO)活性以及过氧化酶 (POD)相对活性 ;用硝酸纤维膜斑点法测定其碱性磷酸酶 (ALP)相对活性 ;用血凝法测定其凝集效价(HAT)。通过对以上免疫指标进行统计分析 ,结果表明 ,WSSV感染与对虾血淋巴PO活性以及ALP相对活性变化有紧密联系 ;不同虾池各免疫因子差异显著 ,发病虾池虾样各免疫指标平均值均低于其他虾池 ;曾发病虾池的虾样PO活性较强 ;WSSV与HPV感染无统计学意义上的相关性 ;未发病虾池与曾发病虾池实验对虾的Ua与UL 相关性极显著 ,发病虾池实验对虾Ua与UL 呈负相关 ;发病虾池对虾PO与ALP活性相关性显著。不同性别中国对虾血淋巴上清液的免疫因子活性没有显著差异 12 9 Penaeus chinensis were caught from the outbreak of white spot syndrome (WSSV virus) shrimp ponds, WSSV outbreak shrimp ponds and WSSV shrimp ponds. Dot blotting and histopathological methods were used to determine the extent of WSSV exposure in each shrimp. The antibacterial activity (Ua), lysozyme activity (UL), phenoloxidase (PO) activity and peroxidase (POD) relative activity of the corresponding individual’s supernatant were measured by 96-well microtiter plate method. Nitrocellulose membrane spots Method to determine the relative activity of alkaline phosphatase (ALP); coagulation titer (HAT) was measured by hemagglutination method. Through the statistical analysis of the above immune indexes, the results showed that the infection of WSSV was closely related to the activity of PO and the relative activity of ALP in shrimp; the immune factors of different shrimp ponds were significantly different, There was no significant correlation between WSSV and HPV infection in other shrimp ponds; the correlation between Ua and UL of unaffected shrimp ponds and shrimp ponds in which shrimp ponds had been infected was extremely significant, The incidence of shrimp pond shrimp Ua and UL was negatively correlated; incidence of shrimp pond shrimp PO and ALP activity was significant. Immunocompetent activity of hemolymph supernatant from shrimp of different sexes was not significantly different