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目的探讨荧光定量PCR检测对诊断和治疗非典型EB病毒(EBV)感染的临床意义。方法对2007-04-2009-10诊断为非典型EBV感染90例患儿,抽静脉血2 ml,注入枸橼酸钠抗凝剂与静脉血充分混匀,用核酸扩增荧光PCR法测定基因拷贝数,同时进行常规外周血及异型淋巴细胞检查,对照病因和首发临床表现,分析儿童感染非典型EBV时荧光定量PCR检测的基因拷贝数变化。结果 EBV-DNA拷贝数能早期反映出非典型EBV感染,拷贝数越高,出现多器官损害概率越高。结论荧光定量PCR检测EBV-DNA拷贝数有助于早期诊断非典型EBV感染,能减少诊疗的盲目性,对临床有一定指导意义。
Objective To investigate the clinical significance of fluorescence quantitative PCR in the diagnosis and treatment of atypical Epstein-Barr virus (EBV) infection. Methods Ninety children diagnosed with atypical EBV infection during 2007-04-2009-10, 2 ml of venous blood were infused, and sodium citrate anticoagulants were injected into the venous blood to mix them thoroughly. The gene was amplified by fluorescent PCR Copy number, at the same time routine peripheral blood and atypical lymphocytes examination, control etiology and initial clinical manifestations, analysis of children infected with atypical EBV fluorescence quantitative PCR detection of gene copy number changes. Results EBV-DNA copy number could reflect atypical EBV infection at early stage. The higher the copy number, the higher the probability of multiple organ damage. Conclusion The detection of EBV-DNA copy number by fluorescence quantitative PCR is helpful to early diagnosis of atypical EBV infection, which can reduce the blindness of diagnosis and treatment, and is of clinical significance.