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目的探讨LTD蛋白在体外对HeLa细胞毒性的影响。方法将不同浓度的LTD蛋白作用于HeLa细胞,光学显微镜下观察细胞形态等变化,MTT法检测细胞增殖情况,激光共聚焦显微镜观察蛋白在细胞内的定位,HE染色和TUNNEL试剂盒检测细胞凋亡情况。结果 LTD蛋白对HeLa细胞具有毒性作用,能抑制细胞增殖,引起细胞凋亡。随着蛋白浓度的增加及作用时间的延长,HeLa细胞凋亡现象明显,生长抑制作用增强,蛋白浓度与细胞A_(490)值之间明显相关;且LTD由定位于细胞膜变为定位于胞浆和细胞核。结论 LTD蛋白促进HeLa细胞凋亡,对其增殖具有明显的抑制作用。
Objective To investigate the effect of LTD protein on HeLa cytotoxicity in vitro. Methods Different concentrations of LTD protein were applied to HeLa cells. The morphological changes of cells were observed under optical microscope. The proliferation of cells was detected by MTT assay. The protein localization in cells was observed by confocal microscopy. The apoptosis of HeLa cells was detected by HE staining and TUNEL. Happening. Results LTD protein has a toxic effect on HeLa cells, can inhibit cell proliferation, causing apoptosis. With the increase of protein concentration and the prolongation of action time, the apoptosis of HeLa cells was obvious and the growth inhibition was enhanced. The concentration of protein was significantly correlated with the value of A 490, and the localization of LTD was localized in the cytoplasm And the nucleus. CONCLUSION LTD protein can promote the apoptosis of HeLa cells and have a significant inhibitory effect on the proliferation of HeLa cells.