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目的：探素神经胶质细胞及人脑胶质瘤细胞是否能分泌β－ｔｐ。方法：用ＥＬＩＳＡ方法分析了胶质瘤病人脑脊液中和细胞培养液中β－ｔｐ水平。结果：３种胶质细胞瘤和患者脑脊液中均能测得β－ｔｐ，其浓度是正常人的１０～１２倍。培养的大鼠胶质细胞随细胞数增加而β－ｔｐ水平增高。在胶质瘤细胞株（ＳＨＧ－４４，Ｕ－２５１，ＢＴ－３２５）培液中β－ｔｐ浓度与细胞增殖的增加成正比。但在非胶质瘤细胞株，如卵巢癌细胞、神经母细胞瘤细胞及前列腺瘤细胞株等，β－ｔｐ的浓度与细胞增殖的趋势相反。结论：胶质细胞和人脑胶质瘤细胞能分泌β－ｔｐ，细胞分泌β－ｔｐ水平的高低由细胞类型及细胞的多寡决定。
Objective: To explore whether β-tp can be secreted by glial cells and human glioma cells. Methods: The level of β-tp in cerebrospinal fluid and cell culture medium of gliomas was analyzed by ELISA. Results: β-tp was detected in cerebrospinal fluid in all three glioblastomas and their patients. The concentration of β-tp in cerebrospinal fluid was 10-12 folds higher than that in normal people. The cultured rat glial cells increased with the cell number and the β-tp level increased. The concentration of β-tp in cultured glioma cell lines (SHG-44, U-251, BT-325) is proportional to the increase in cell proliferation. However, in non-glioma cell lines, such as ovarian cancer cells, neuroblastoma and prostate cancer cell lines, the concentration of β-tp is opposite to that of cell proliferation. Conclusion: Glial cells and human glioma cells can secrete β-tp, and the level of β-tp secretion is determined by cell type and cell number.