论文部分内容阅读
目的 :探讨食道鳞癌DNA含量定量分析的临床意义。方法 :选择 45例手术根治切除食道鳞癌标本 ,应用图像细胞计技术测量肿瘤细胞DNA含量和倍体 ,与正常食道鳞状上皮对照 ,瘤体大小 (直径 >3cm或≤ 3cm) ,有无淋巴结转移 ,病理组织学分级与DNA含量对照。结果 :正常食道鳞状上皮与食道鳞癌DNA含量存在显著差异 (P<0 .0 1) ,瘤体大小的平均DNA含量存在差异 (P <0 .0 5 ) ,DNA含量与有无淋巴结转移有显著差异 (P <0 .0 5 ) ,病理组织学分级Ⅰ级与Ⅲ级存在显著差异 (P <0 .0 5 ) ,Ⅱ级与Ⅲ级无显著差异 (P >0 .0 5 )。结论 :DNA含量定量分析在食道鳞癌恶性生物学行为中具有重要价值 ,是临床治疗和预后估计的有用指标
Objective: To explore the clinical significance of quantitative analysis of DNA content in esophageal squamous cell carcinoma. METHODS: Forty-five patients undergoing radical resection of esophageal squamous cell carcinoma were studied. Image cytometry was used to measure DNA content and ploidy of tumor cells, compared with normal esophageal squamous epithelium, tumor size (>3cm or ≤ 3cm in diameter), and lymph nodes. Metastasis, histological grade and DNA content were compared. RESULTS: There was a significant difference in DNA content between normal esophageal squamous epithelium and esophageal squamous cell carcinoma (P < 0.01). The average DNA content of the tumor size was different (P <0.05), DNA content and lymph node metastasis. There was a significant difference (P < 0.05). There was a significant difference (P <0.05) between histological grade I and III, and there was no significant difference between grade II and III (P> 0.05). Conclusion: Quantitative analysis of DNA content is of great value in malignant biological behaviors of esophageal squamous cell carcinoma and is a useful indicator for clinical treatment and prognosis estimation.