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Background Little information is available regarding the effect of angiotensin Ⅱ (Ang Ⅱ) on the bumetanide-sensitive sodium-potassium-2 chloride cotransporter (NKCC_2), the thiazide-sensitive sodium-chloride cotransporter (NCC), and the Cl- channel (CLC)-K_2 at both mRNA and protein expression level in Ang Ⅱ-induced hypertensive rats. This study was conducted to investigate the influence of Ang Ⅱ with chronic subpressor infusion on nephron-specific gene expression of NKCC_2, NCC and CLC-K_2. Methods Sprague Dawleys rats were treated subcutaneously with either Ang Ⅱ (100 ng·kg -1·min -1 ) or vehicle for 14 days. Expression of NKCC_2, NCC and CLC-K_2 mRNA in kidneys was determined by real time polymerase chain reaction (PCR). Western blotting analysis was used to measure NKCC_2 and NCC protein expression.Results Ang Ⅱ significantly increased blood pressure and up-regulated NKCC_2 mRNA and protein expression in the kidney. Expression of CLC-K_2 mRNA in the kidney increased 1.6 fold (P<0.05).There were no changes in NCC mRNA or protein expression in AngII-treated rats versus control.ConclusionsChronic subpressor Ang Ⅱ infusion can significantly alter NKCC_2 and CLC-K_2 mRNA expression in the kidney, and protein abundance of NKCC_2 in kidney is positively regulated by Ang Ⅱ. These effects may contribute to enhanced renal Na+ and Cl- reabsorption in response to Ang Ⅱ
Background Little information is available regarding the effect of angiotensin II (Ang II) on the bumetanide-sensitive sodium-potassium-2 chloride cotransporter (NKCC_2), the thiazide-sensitive sodium- chloride cotransporter (NCC), and the Cl- channel ) -K_2 at both mRNA and protein expression level in Ang Ⅱ-induced hypertensive rats. This study was conducted to investigate the influence of Ang Ⅱ with chronic subpressor infusion on nephron-specific gene expression of NKCC_2, NCC and CLC-K_2. Methods Sprague Dawley rats were treated subcutaneously with either Ang Ⅱ (100 ng · kg -1 · min -1) or vehicle for 14 days. Expression of NKCC_2, NCC and CLC-K_2 mRNA in kidneys was determined by real time polymerase chain reaction (PCR) . Expression of CLC-K_2 mRNA in the kidney increased 1 .6 fold (P <0.05). Everyone no changes in NCC mRNA or protein expression in AngII-treated rats versus control. ConclusionsChronic subpressor Ang Ⅱ infusion can significantly alter NKCC_2 and CLC-K_2 mRNA expression in the kidney, and protein abundance of NKCC_2 in kidney iswas regulated by Ang Ⅱ. These effects may contribute to enhanced renal Na + and Cl- reabsorption in response to Ang Ⅱ