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目的:观察甘利欣注射液对哮喘大鼠支气管和肺组织形态及嗜酸粒细胞(EOS)数的影响,探讨甘利欣注射液干预哮喘气道炎症的作用机制。方法:清洁级雄性SD大鼠60只,随机分为6组:正常对照组、模型对照组、地塞米松治疗组、大、中、小剂量甘利欣治疗组,每组10只,采用卵蛋白制作大鼠哮喘模型。各组给药2周后检测各组大鼠血清和支气管肺泡灌洗液中EOS数,观察支气管和肺组织的病理形态改变。结果:模型对照组EOS数明显高于正常对照组(P<0.05),支气管上皮损伤及基底膜增厚明显。与模型对照组比较,地塞米松治疗组与大、中剂量甘利欣治疗组均可不同程度的下调EOS数,支气管上皮损伤及基底膜增厚明显减轻。小剂量甘利欣治疗组则无此作用。结论:甘利欣注射液治疗哮喘气道炎症的作用机制可能与减少EOS对肺组织的浸润有关。
Objective: To observe the effect of Ganlixin injection on bronchus and lung tissue morphology and number of eosinophils (EOS) in asthmatic rats, and to explore the mechanism of action of Ganlixin injection in asthmatic airway inflammation. METHODS: Sixty male SD rats of clean grade were randomly divided into 6 groups: normal control group, model control group, dexamethasone treatment group, large, medium, and low dose Ganlixin treatment group, 10 in each group. Rat asthma model was made. The number of EOS in serum and bronchoalveolar lavage fluid was measured in each group 2 weeks after administration, and the pathological changes of bronchus and lung tissues were observed. RESULTS: The number of EOS in the model control group was significantly higher than that in the normal control group (P<0.05). Bronchial epithelium injury and basement membrane thickening were evident. Compared with the model control group, the dexamethasone treatment group and the large and medium dose Ganlixin treatment group can reduce the number of EOS to varying degrees, and the bronchial epithelial injury and basement membrane thickening are significantly reduced. The low-dose Ganlixin treatment group did not have this effect. Conclusion: The mechanism of action of Ganlixin in treating asthmatic airway inflammation may be related to reducing the infiltration of lung tissue by EOS.