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目的:探讨宫颈上皮内瘤变(Cervical Intraepithelial Neoplasias,CIN)及宫颈癌组织中P16INK4A、Ki-67的表达及与HPV16/18感染的关系。方法:选取宫颈组织石蜡标本94例,包括慢性宫颈炎20例,CINⅠ13例,CINⅡ17例,CINⅢ20例和宫颈癌24例,用免疫组化法检测P16INK4A、Ki-67的表达及PCR技术检测HPV16/18型DNA。结果:①P16INK4A在慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组及宫颈癌组阳性表达率分别是0.0%、53.8%、76.5%、95.0%、100.0%,CINⅢ组及宫颈癌组与其他各组比较,差异有统计学意义(P<0.05);②Ki-67在慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组及宫颈癌组阳性表达率分别是0.0%、69.2%、88.2%、95.0%、91.7%,CIN组和宫颈癌组与慢性炎症组比较,差异有统计学意义;③HPV16/18主要感染高级别CIN及宫颈癌;④P16INK4A及Ki-67的表达强度与宫颈病变程度呈正相关,并且与HPV16/18型感染也呈正相关。结论:HPV16/18、P16INK4A、Ki-67与宫颈癌的发生、发展有一定关系。
Objective: To investigate the expression of P16INK4A and Ki-67 in cervical intraepithelial neoplasia (CIN) and cervical cancer and its relationship with HPV16 / 18 infection. Methods: 94 cases of cervical tissue paraffin were selected, including 20 cases of chronic cervicitis, 13 cases of CINⅠ, 17 cases of CINⅡ, 20 cases of CINⅢ and 24 cases of cervical cancer. The expressions of P16INK4A and Ki-67 were detected by immunohistochemistry and HPV16 / 18 DNA. Results: ① The positive rates of P16INK4A in chronic cervicitis group, CINⅠgroup, CINⅡ group, CINⅢ group and cervical cancer group were 0.0%, 53.8%, 76.5%, 95.0% and 100.0% (P <0.05). ② The positive rates of Ki67 in chronic cervicitis group, CINⅠgroup, CINⅡ group, CINⅢ group and cervical cancer group were 0.0%, 69.2%, 88.2%, 95.0 %, 91.7%. There was significant difference between CIN group and cervical cancer group and chronic inflammation group. (3) HPV16 / 18 mainly infected high grade CIN and cervical cancer. (4) The expression of P16INK4A and Ki-67 was positively correlated with the degree of cervical lesions, And with HPV16 / 18 infection was also positively correlated. Conclusion: HPV16 / 18, P16INK4A, Ki-67 and cervical cancer occurrence and development have a certain relationship.