目的 :报导短程大剂量格拉诺赛特 (rhG CSF)动员外周血造血干细胞 ,进行自体外周血造血干细胞移植 (APBSCT)治疗急性白血病。方法 :rhG CSF ,5 0 0 μg/d皮下注射 4天 ,第 5、6天单采外周血单个核细胞 (MNC) ,行CFU GM培养 ,CD34+细胞由流式细胞仪检测。予MAC方案处理 ,回输自体外周围血造血干细胞 (APBSC)。结果 :动员后CFU GM明显增高 ,CD34+细胞增多 ,骨髓造血功能重建迅速。结论 :短程大剂量格拉诺赛特在促进骨髓造血功能重建 ,具有重要作用 OBJECTIVE : To report that peripheral blood hematopoietic stem cells were mobilized by short-range high-dose GranoSite (rhG CSF) and autologous peripheral blood stem cell transplantation (APBSCT) for acute leukemia. METHODS: RhG CSF was subcutaneously injected at 500 μg/d for 4 days, and peripheral blood mononuclear cells (MNCs) were harvested on days 5 and 6 for CFU GM culture. CD34+ cells were detected by flow cytometry. For the MAC protocol treatment, reinfusion of peripheral blood hematopoietic stem cells (APBSC). Results: CFU GM increased significantly after mobilization, CD34+ cells increased, and bone marrow hematopoietic function reconstructed rapidly. Conclusion: Short-course large-dose Granosite plays an important role in promoting bone marrow hematopoietic function reconstruction