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目的 :探讨胎儿卵巢的体外保存方法。方法 :将 16例 2 0～ 2 8周的胎儿卵巢置于含 1.41mol/L的二甲基亚砜 (DMSO)培养液中 ,采用三步降温法投入液氮保存 ,复苏时采用 40℃水浴快速复温和室温下慢复温 ,然后进行组织培养并观察卵泡发育和雌二醇 (E2 )分泌情况。结果 :胎儿卵巢冻存后复苏培养 ,其卵泡仍能继续发育并产生E2 ;快速复温组卵泡组织形态良好 ,并能维持良好的内分泌功能 ;慢复温组卵巢上皮破裂 ,E2 分泌量明显减少。结论 :“三步降温法”冻存胎儿卵巢效果好 ,快速复温能更好地维持卵泡形态发育和内分泌功能
Objective: To investigate the method of in vitro preservation of fetal ovary. Methods: Twenty-six cases of fetus ovariectomies ranging from 20 to 28 weeks were housed in dimethyl sulfoxide (DMSO) medium containing 1.41mol / L, and were stored in liquid nitrogen by the three-step cooling method. When the temperature was 40 ℃, Rapid rewarming and slow rewarming at room temperature, followed by tissue culture and observation of follicular development and estradiol (E2) secretion. RESULTS: Fetal ovary survived after cryopreservation, and its follicles could continue to develop and produce E2. The ovarian follicles in fast rewarming group had good morphology and maintained good endocrine function. The ovarian epithelial ruptured and the secretion of E2 decreased significantly . Conclusion: The “three-step cooling method” has a good effect on cryopreservation of fetal ovary, and rapid rewarming can better maintain the development of follicular morphology and endocrine function