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目的 :比较胎儿卵巢、成人卵巢免疫原性的差异 ,并观察培养前后胎儿卵巢免疫原性的变化。方法 :对培养前后胎儿卵巢及成人卵巢的冰冻切片用人白细胞抗原 (HLA DR)单克隆抗体进行免疫组织化学染色 ,观察其阳性细胞形态及分布并计数。结果 :胎儿卵巢内HLA DR抗原含量显著少于成人卵巢 (P <0 .0 0 1) ,且培养后胎儿卵巢HLA DR抗原含量显著低于培养前 (P <0 .0 1) ,随培养天数的增加HLA DR抗原含量逐渐减少 (P <0 .0 1)。结论 :胎儿卵巢的免疫原性低于成人卵巢 ,且培养后进一步降低。以培养一段时间的胎儿卵巢作为移植供体 ,可能提高临床卵巢移植的成功率。
OBJECTIVE: To compare the immunogenicity of ovary and adult ovaries and to observe the changes of immunogenicity of fetus before and after culture. Methods: The frozen sections of fetal ovary and adult ovary before and after culture were immunohistochemically stained with monoclonal antibody against human leukocyte antigen (HLA DR), and the morphology and distribution of positive cells were observed. Results: The content of HLA DR antigen in fetal ovary was significantly lower than that in adult ovary (P <0.01), and the content of HLA DR antigen in fetal ovary after culture was significantly lower than that before culturing (P <0.01) Increased the level of HLA DR antigen gradually decreased (P <0.01). Conclusion: The immunogenicity of fetal ovary is lower than that of adult ovary and further decreased after cultured. To raise fetal ovaries for a period of time as a transplant donor, may improve the success rate of clinical ovarian transplantation.