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目的:克隆黄芩延长因子1a全长cDNA序列,并分析温度对其表达水平的影响。方法:通过构建黄芩全长cDNA文库克隆黄芩延长因子1a,利用生物信息学手段分析该基因的序列特征;利用RT-PCR分析温度对黄芩延长因子1a基因表达水平的影响。结果:黄芩延长因子1a基因长1 672 bp,开放阅读框位于97～1 446,共449个氨基酸,具有典型的延长因子1a结构域;高温和低温处理后,黄芩EF1a的转录水平均显著提高。结论:黄芩EF1a的克隆为进一步研究其在黄芩生长代谢过程中的功能和作用提供了基础。
OBJECTIVE: To clone full-length cDNA of elongation factor 1a from Scutellaria baicalensis Georgi and analyze the effect of temperature on its expression. Methods: The cDNA library of Scutellaria baicalensis was constructed by cloning Scutellaria baicalensis lengthening factor 1a. The sequence characteristics of Scutellaria baicalensis were analyzed by bioinformatics methods. The effect of temperature on the gene expression of Scutellaria baicalensis elongin-1a was analyzed by RT-PCR. RESULTS: The elongation factor 1 a gene of Scutellaria baicalensis was 1 672 bp in length and the open reading frame (ORF) was located at 97-1446, a total of 449 amino acids with a typical elongation factor 1a domain. The transcription level of EF1a in Scutellaria baicalensis Georgi was significantly increased after high temperature and low temperature treatment. Conclusion: The cloning of Scutellaria baicalensis EF1a provides a basis for further study of its function and role in the growth and metabolism of Scutellaria baicalensis Georgi.