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目的以肾血管性高血压大鼠模型为高血病背景,研究在此模型基础上使用胶原酶和肝素脑内尾状核定点注射建立脑出血模型的方法。方法高血压大鼠模型先使用双肾单夹法来初步建立,20周后注射胶原酶和肝素脑内尾状核建立脑出血大鼠模型。脑出血大鼠模型使用不同药物浓度配伍和注射剂量的结果分别进行了2、24、48h,1个月的整体观察和显微镜下结构形态学观察,并就在脑出血模型制作中正常大鼠模型和肾血管性高血压大鼠模型的神经行为学,死亡率,对脑出血耐受力及自然恢复力差异进行分析。结果在第1次手术4周后,实验组血压从(110±4)mmHg升至(164±6)mmHg(P<0.01),20周后基本稳定在(178±6)mmHg。第2次手术后2h,形成直径3mm的血肿区,术后24h基本形成直径3mm的血凝块。注射药物配伍胶原酶0.5U/μl,肝素为5U/μl,注射剂量为0.8μl的实验组具有死亡率较低(2/30),神经行为学得分改变较小(由急性期的7.8±0.8到1个月时维持在8.5±0.5)的特点。结论用肾血管性高血压大鼠建模较用正常大鼠建模模拟性更好,神经行为学改变的结果更稳定、持久,受自然恢复的影响更小。
OBJECTIVE: To establish a model of intracerebral hemorrhage by using collagenase and heparin in the caudate nucleus of the rat as a model of hypertensive disease in a renovascular hypertensive rat model. Methods Hypertensive rat model was established by double-kidney single-clip method. After 20 weeks, collagenase and heparinized caudate nucleus were injected into the rat model of intracerebral hemorrhage. The cerebral hemorrhage rat model using different concentrations of drug compatibility and injection dose results were observed for 2, 24, 48h, 1 month and microscopic morphological observation, and in the model of intracerebral hemorrhage in normal rat model And renal vascular hypertensive rats model of neurobehavioral, mortality, tolerance to cerebral hemorrhage and natural resilience were analyzed. Results After 4 weeks of the first operation, the blood pressure increased from (110 ± 4) mmHg to (164 ± 6) mmHg in the experimental group (P <0.01), and remained stable at (178 ± 6) mmHg after 20 weeks. 2h after the second operation, a hematoma area of 3mm in diameter was formed, and a blood clot of 3mm in diameter was formed basically 24 hours after the operation. The experimental group with injection of 0.5 U / μl collagenase, 5 U / μl heparin and 0.8 μl injection had lower mortality (2/30) and less neurobehavioral change (from 7.8 ± 0.8 in acute phase To 1 month to maintain the characteristics of 8.5 ± 0.5). Conclusion The model of renovascular hypertensive rats is better than that of normal rats. The results of neurobehavioral changes are more stable and durable, and are less affected by natural recovery.