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Objective:To investigate the susceptibility of strains separated from probiotic products for medical purpose to 14 antimicrobial agents. Methods:The single aerobic strains were isolated from these products respectively and disc agar diffusion assay was proceeded to determine the susceptibility. Results:Probiotics tested in the study mostly showed multiresistant to the agents. Lactobacillus acidophilus LAP,LAB, Lactobacillus bulgaricus LBJ and Streptococcus thermophilus STJ were resistant to vancomycin. Conclusion: Drug resistance exists in most of commercial probiotics. The evaluation and monitoring of safety of probiotic products for medical purpose should be paid great attention.