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目的 探讨 CD44 v6在胃癌及其淋巴结转移灶中的表达及其临床意义。方法 应用免疫组织化学链霉菌抗生物素蛋白—过氧化物酶 (SP)法检测 3 0名正常人胃组织 ,69例胃癌患者原发灶及其 42例淋巴结转移灶中 CD44 v6的表达。结果 正常胃组织均显示清楚的细胞膜染色。胃癌原发灶及淋巴结转移灶中 CD44 v6表达阳性率分别为 5 6.5 % (3 9/ 69)和 66.7% (2 8/ 42 ) ,两者之间差异无显著性 (P>0 .0 5 ) ,但均与正常胃组织差异有显著性 (P<0 .0 0 1)。CD44 v6的表达与胃癌组织类型无关 (P>0 .0 5 ) ,但和 P- TNM分期、病理分级及淋巴结转移密切相关 (P均 <0 .0 5 )。结论 检测 CD44 v6基因的表达可作为判定胃癌的生物学行为 ,为预测胃癌的转移以及评估预后提供依据
Objective To investigate the expression of CD44 v6 in gastric cancer and its lymph node metastasis and its clinical significance. Methods Immunohistochemical streptavidin-peroxidase (SP) method was used to detect the expression of CD44v6 in 30 normal gastric tissues and 69 cases of gastric cancer and 42 cases of lymph node metastasis. Results Normal gastric tissue showed clear cell membrane staining. The positive rates of CD44 v6 expression in primary gastric cancer and lymph node metastasis were 56.5% (39/69) and 66.7% (28/42), respectively, with no significant difference (P> 0.05) ), But both were significantly different from normal gastric tissues (P <0.01). The expression of CD44v6 was not related to the type of gastric cancer (P> 0.05), but closely related to the stage of P-TNM, pathological grade and lymph node metastasis (all P <0.05). Conclusion Detecting the expression of CD44 v6 gene can be used as a basis for determining the biological behavior of gastric cancer and providing a basis for predicting the metastasis of gastric cancer and evaluating the prognosis