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目的建立一种鉴别检测苹果汁掺假情况的元素分析/液相色谱-同位素比值质谱法(elemental analysis/liquid chromatography-isotope ratio mass spectrometry,EA/LC-IRMS)。方法通过对不同产区不同种类118个纯正苹果汁的糖、有机酸、果糖、葡萄糖、二糖、寡糖碳同位素比值(δ~(13)C值)的测定,建立纯正苹果汁同位素数据库进而提出纯正苹果汁应满足的δ~(13)C值要求。结果有机酸和糖差值Δδ~(13)CO-S在-1.38‰至1.09‰范围内,果糖和葡萄糖差值Δδ~(13)CF-G在-0.70‰至0.57‰范围内,而各组分最大差值Δδ~(13)Cmax<4.36‰。对市售105个苹果汁进行检测,采用本方法检出32个阳性样品,而采用本实验室的糖浆标志物法仅检出12个阳性样品。结论本方法大大提高了苹果汁的掺假鉴别,有很大的实际应用潜力。
Objective To establish an elemental analysis / liquid chromatography-isotope ratio mass spectrometry (EA / LC-IRMS) for the identification of adulteration of apple juice. Methods The pure apple juice isotopic database was established by measuring the carbon isotope ratio of organic acids, fructose, glucose, disaccharides and oligosaccharides (δ ~ (13) C) of 118 apple cider apples from different regions. Put forward the pure apple juice should meet the δ ~ (13) C value requirements. The results showed that the difference of Δδ ~ (13) CO-S between organic acid and sugar was in the range of -1.38 ‰ to 1.09 ‰, and the difference of Δδ ~ (13) CF-G between fructose and glucose was within -0.70 ‰ to 0.57 ‰, The maximum difference of the components Δδ ~ (13) Cmax <4.36 ‰. A total of 105 apple juice samples were tested and 32 positive samples were detected by this method. Only 12 positive samples were detected by the syrup marker method in our laboratory. Conclusion This method greatly enhances the identification of apple juice adulteration, has great practical potential.