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基底的性质和制备方法对吸附胶原层的超分子自组装过程有很大的影响,将一种Gemini表面活性剂C18H37(CH3)2N+-(CH2)12-N+(CH3)2C18H37·2Br-(简写为18-12-18)利用LB技术在零压下转移到云母片上制成基底,浸入到pH值为3.0的胶原溶液中37℃恒温吸附不同的时间,样品用原子力显微镜(AFM)进行表征。结果表明:在恒温过程中,LB膜上的表面活性剂分子会发生移动和翻转,胶原分子优先吸附在LB膜上形成单层网状结构,随吸附时间的延长,胶原分子也会在云母上发生吸附,最后两者会交织成无序的胶原纤维。
The properties and preparation of the substrate have a great influence on the superamolecular self-assembly of the adsorbed collagen layer. A Gemini surfactant C18H37 (CH3) 2N + - (CH2) 12-N + (CH3) 2C18H37.2Br- 18-18-18) was transferred to mica by zero pressure technique to fabricate a substrate. The gel was immersed in a collagen solution at pH 3.0 for different times at 37 ° C. The samples were characterized by atomic force microscopy (AFM). The results show that the surfactant molecules on the LB film will move and flip during the thermostatic process. The collagen molecules preferentially adsorb on the LB film to form a monolayer network structure. With the extension of the adsorption time, the collagen molecules will also be on the mica Adsorption, the last two will be interwoven into disordered collagen fibers.