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目的探讨人脐带间充质干细胞(UC-MSCs)移植修复烧伤皮肤的机制。方法 24只SD大鼠均分为UC-MSCs移植治疗组和对照组。分别收集烧伤后12h、1、3、7d两组大鼠损伤局部的皮肤组织,抽提蛋白;ELISA法测定皮肤组织内细胞因子TNF-α、IL-1及IFN-γ的表达,Western blot法检测大鼠皮肤中MCP-1及CCX-2蛋白的变化,免疫组化法检测烧伤后14d两组碱性成纤维细胞生长因子(bFGF)及血管内皮生长因子(VEGF)表达量。结果烧伤后,大鼠烧伤皮肤细胞因子TNF-α和IL-1增加,IFN-γ表达下降;治疗组UC-MSCs移植后3d,TNF-α和IL-1下降,IFN-γ含量增加(P<0.05);烧伤7d时,受损皮肤中趋化因子MCP-1及CCX-2的表达增加,而UC-MSCs移植治疗组在烧伤7d时受损皮肤中MCP-1及CCX-2的表达量下降(P<0.05)。烧伤14d时,UC-MSCs移植治疗组的bFGF及VEGF表达量较对照组增加(P<0.05)。结论烧伤后,UC-MSCs移植治疗可能通过抑制皮损局部的炎症反应,减少淋巴细胞的浸润,促进血管的再生和成纤维细胞的生长来促进损伤皮肤的修复。
Objective To investigate the mechanism of human umbilical cord mesenchymal stem cell (UC-MSCs) transplantation for the repair of burn skin. Methods Twenty-four SD rats were divided into UC-MSCs transplantation group and control group. Skin tissue and protein extracted from injured rats were harvested at 12h, 1,3,7d after burn respectively. The expressions of TNF-α, IL-1 and IFN-γ in skin tissue were detected by ELISA. Western blot The changes of MCP-1 and CCX-2 protein in the skin of rats were detected. The expression of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in the two groups were detected by immunohistochemical method 14 days after burn. Results After burn, the levels of cytokines TNF-α and IL-1 increased and the expression of IFN-γ decreased in the burn skin. The levels of TNF-α and IL-1 decreased and the content of IFN-γ increased <0.05). The expressions of chemokines MCP-1 and CCX-2 in the damaged skin increased at 7 d after burn, while the expression of MCP-1 and CCX-2 in the damaged skin of UC-MSCs transplanted group at 7 d after burn Decreased (P <0.05). On the 14th day after burn, the expression of bFGF and VEGF in UC-MSCs transplantation group was higher than that in control group (P <0.05). Conclusion UC-MSCs transplantation may promote the repair of damaged skin by inhibiting inflammation in the lesion, reducing lymphocyte infiltration, promoting blood vessel regeneration and fibroblast growth.