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目的探讨番茄红素对肠缺血再灌注大鼠肠黏膜的保护作用及机制。方法健康清洁级SD大鼠48只按随机数字表法分为番茄红素组、缺血再灌注组、假手术组,每组16只。番茄红素组予以番茄红素10 mg/kg连续灌胃5 d,缺血再灌注组和假手术组灌胃相同剂量生理盐水。各组大鼠分离肠系膜上动脉,番茄红素组和缺血再灌注组以血管夹夹闭肠系膜上动脉1 h,再灌注4 h后,各组大鼠采集腹主动脉血和回肠标本,测定血清超氧化物歧化酶(superoxide dismutase,SOD)、二胺氧化酶(diamine oxidase,DAO)活性和丙二醛(malondialdehyde,MDA)含量,小肠组织髓过氧化物酶(myeloperoxidase,MPO)活性及肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)与白细胞介素6(interleukin-6,IL-6)的含量,以及小肠组织病理学Chiu’s评分。结果番茄红素组小肠组织病理学Chiu’s评分明显低于缺血再灌注组(P<0.01),但与假手术组相比差异仍有统计学意义(P<0.01)。番茄红素组小肠组织TNF-α、IL-6、MPO及血清SOD、DAO和MDA明显优于缺血再灌组(P<0.01),而小肠组织TNF-α、IL-6、MPO及血清DAO、SOD、MDA与假手术组比较差异仍有统计学意义(P<0.01)。结论番茄红素预处理能有效抑制肠缺血再灌注后的氧化应激反应及炎症反应,减轻小肠缺血再灌注损伤。
Objective To investigate the protective effect of lycopene on intestinal mucosa in rats with intestinal ischemia-reperfusion and its mechanism. Methods Forty-eight healthy SD rats were randomly divided into three groups: lycopene group, ischemia reperfusion group and sham operation group, 16 rats in each group. The lycopene group was given lycopene 10 mg / kg for 5 days. The same dose of saline was given to the ischemia reperfusion group and the sham operation group. The superior mesenteric artery, lycopene group and ischemia-reperfusion group were divided into two groups: the superior mesenteric artery was occluded by vascular clamp for 1 h and the reperfusion for 4 h, the abdominal aorta blood and ileum samples were collected from each group. The activities of superoxide dismutase (SOD), diamine oxidase (DAO) and malondialdehyde (MDA), the activities of myeloperoxidase (MPO) The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), as well as histopathological Chiu’s score of small intestine, were measured. Results The small intestine histopathology Chiu’s score of lycopene group was significantly lower than that of ischemia-reperfusion group (P <0.01), but there was still significant difference between the two groups (P <0.01). The levels of TNF-α, IL-6, MPO, SOD, DAO and MDA in small intestine of lycopene group were significantly higher than those of ischemia-reperfusion group (P <0.01) The difference of DAO, SOD, MDA and sham group was still statistically significant (P <0.01). Conclusions Lycopene preconditioning can effectively inhibit the oxidative stress and inflammation after intestinal ischemia / reperfusion and relieve the intestinal ischemia reperfusion injury.