在食用菌生产中，制种是最关键的一环。我们在菌研工作中，经过近5年的探索和改进，取得了良好的效果。一、组织分离法制种改进技术常规组织分离法，是将选好的优良种菇，在无菌条件下用70％酒精或0.1％升汞水表面消毒，切取0.2－0.4cm3组织接入斜面培养基试管中，置于恒温箱培 In mushroom production, seed production is the most crucial part. We have achieved good results in the research of bacteria research after nearly five years of exploration and improvement. First, the organizational separation of seed production of improved technology Conventional tissue separation is selected good mushrooms, sterile conditions with 70% alcohol or 0.1% mercury surface water disinfection, cut 0.2-0.4cm3 tissue access bevel culture Base tube, placed in incubator training