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目的 :分别检测胃癌、癌旁和正常胃组织中DNA含量 ,分析DNA含量与胃癌临床病理特征的关系。方法 :对36例胃癌组织、30例相应癌旁组织、2 5例正常胃组织 ,采用流式细胞术检测细胞核DNA含量。结果 :在胃癌、癌旁和正常胃组织中 ,细胞核DNA含量分别为 1 39± 0 2 3、1 0 0± 0 0 5和 0 99± 0 0 5 ,胃癌组织的DNA含量明显高于癌旁及正常胃组织 (P <0 0 1) ,而癌旁组织与正常胃组织的DNA含量相似 (P >0 0 5 )。分化程度差 ,深度浸润 ,伴淋巴结转移及Ⅲ、Ⅳ期的胃癌组织 ,其DNA含量明显高于分化程度较好 ,浸润较浅 ,不伴淋巴结转移及Ⅰ、Ⅱ期的胃癌组织。结论 :DNA含量可作为判断胃癌浸润、转移及预后的一个良好的指标。
Objective: To detect the content of DNA in gastric cancer, paracancer and normal gastric tissue respectively, and to analyze the relationship between DNA content and clinicopathological features of gastric cancer. Methods: DNA was detected by flow cytometry in 36 cases of gastric carcinoma, 30 cases of corresponding paracancerous tissues and 25 cases of normal gastric tissues. Results: In gastric cancer, paracancer and normal gastric tissue, the content of DNA in the nucleus was significantly higher than that in the adjacent tissues and the normal gastric tissues respectively (399 ± 0 2 3, 100 ± 0 05 and 0 99 ± 0 05) Normal gastric tissue (P <0.01), while the adjacent non-cancerous tissue and normal gastric tissue DNA content was similar (P> 0.05). Poorly differentiated, deep infiltration, with lymph node metastasis and stage Ⅲ, Ⅳ gastric cancer tissue DNA content was significantly higher than the well differentiated, less infiltrated, without lymph node metastasis and Ⅰ, Ⅱ gastric cancer. Conclusion: DNA content can be used as a good indicator of gastric cancer invasion, metastasis and prognosis.