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目的研究一氧化氮(NO)对豚鼠耳蜗外毛细胞钙通道的影响。方法以硝普钠作为NO的供体,应用全细胞式膜片钳技术记录外毛细胞钙电流。结果 10μmol/L硝普钠使电压依赖性钙通道电流减小,电流电压关系(I-V)曲线上抬;0.05～50μmol/L硝普钠对电流的抑制率与其浓度有关,半数抑制浓度为0.79μmol/L,对电流的最大抑制率为39.48%,其阻断作用是可逆的。结论硝普钠可阻断豚鼠耳蜗外毛细胞电压依赖性钙通道;推测NO在传出神经对外毛细胞功能调节中起重要作用。
Objective To study the effect of nitric oxide (NO) on the calcium channel of guinea pig cochlear outer hair cells. Methods Sodium nitroprusside was used as donor of NO. Total cell patch clamp technique was used to record the outer hair cell calcium current. The results showed that 10 μmol / L sodium nitroprusside decreased voltage-dependent calcium channel current and the current-voltage relationship (IV) curve was up-regulated. The inhibitory rate of current with 0.05-50 μmol / L sodium nitroprusside was related to its concentration, / L, the maximum inhibition rate of current is 39.48%, its blocking effect is reversible. Conclusion Sodium nitroprusside can block the voltage-dependent calcium channels of cochlear outer hair cells in guinea pigs. It is speculated that NO plays an important role in regulating the function of the outer hair cells.