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目的建立UPLC同时测定复方守宫散中腺嘌呤、腺苷含量的方法。方法色谱柱Acquity BEH C18(2.1 mm×100 mm,1.7μm),甲醇-水为流动相,梯度洗脱,流速:0.25 mL·min-1,柱温:26℃,检测波长:254 nm。结果腺嘌呤、腺苷的进样量分别在0.040 9~0.511 5 mg·mL-1(r=0.999 9)和0.057 8~0.722 0 mg·mL-1(r=0.999 6)内与其峰面积呈良好的线性关系;平均回收率分别为97.7%,97.7%,RSD分别为0.96%,1.18%。结论该方法快速、可靠、准确,可作为复方守宫散的质量控制方法。
Objective To establish a method for the simultaneous determination of adenosine and adenosine in Fufang Shengong Powder by UPLC. Methods The mobile phase was Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm). The mobile phase consisted of methanol and water with a gradient of 0.25 mL · min-1. The column temperature was 26 ℃ and the detection wavelength was 254 nm. Results The peak area of adenine and adenosine in the range of 0.040 9-0.511 5 mg · mL-1 (r = 0.999 9) and 0.057 8-0.722 0 mg · mL-1 (r = 0.999 6) Good linear relationship; average recovery rate was 97.7%, 97.7%, RSD were 0.96%, 1.18%. Conclusion The method is rapid, reliable and accurate and can be used as a quality control method for compound prescription.