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目的:研究过表达电压门控氯通道家族蛋白成员3(voltage-gated chloride channel family protein 3,ClC-3)基因对小鼠骨骼的影响。方法:3月龄雄性FVB小鼠用鼠尾基因检测法确定基因型后分为2组:野生型(WT)组和ClC-3过表达(ClC-3 transgene)组,每组各8只。分别测量2组小鼠体重,右侧胫骨长度和重量。取胫骨上段和中段进行脱钙,石蜡包埋,切片,HE染色后,采用骨形态计量学分析胫骨上段松质骨的骨小梁面积百分数(percent trabecular area,%Tb.Ar)、骨小梁数量(trabecular number,Tb.N)、骨小梁宽度(trabecular width,Tb.Wi)、骨小梁分离度(trabecular separation,Tb.Sp)和胫骨中段皮质骨的骨组织总面积(total tissue area,T.Ar)、皮质骨面积(cortical area,Ct.Ar)、皮质骨面积百分数(percent cortical area,%Ct.Ar)、骨髓腔面积(marrow area,Ma.Ar)、骨髓腔面积百分数(percent marrow area,%Ma.Ar)。结果:小鼠经鼠尾基因检测后确认为野生型或ClC-3过表达型。与WT组相比,ClC-3 transgene组小鼠体重及胫骨长度重量均减小(P<0.05);松质骨的%Tb.Ar和Tb.Wi均减小(P<0.05),Tb.Sp增大(P<0.05),Tb.N的变化无统计学意义;皮质骨的T.Ar、Ct.Ar和%Ct.Ar均减小(P<0.05),%Ma.Ar增大(P<0.05),Ma.Ar的变化无统计学意义。结论:ClC-3过表达导致小鼠的皮质骨和松质骨骨量减少,骨结构变差,提示ClC-3可能参与了骨形成和/或骨吸收。
AIM: To investigate the effect of over-expression of voltage-gated chloride channel family protein 3 (ClC-3) on mouse bone. Methods: Three-month-old male FVB mice were genotyped by murine tail gene assay and divided into 2 groups: WT group and ClC-3 transgene group, 8 in each group. Body weight, right tibia length and weight of the two groups were measured respectively. The upper and middle tibias were decalcified, paraffin-embedded, sectioned and stained with hematoxylin and eosin (HE). Bone morphometry was used to analyze the percent trabecular area (% Tb.Ar) Trabecular number (Tb.Wi), trabecular separation (Tb.Sp) and total tissue area of cortical bone in the middle part of the tibia (total bone area , T.Ar), cortical area (Ct.Ar), percent cortical area (% Ct.Ar), marrow area (Ma.Ar), bone marrow cavity area percentage percent marrow area,% Ma.Ar). Results: Mice were confirmed as wild-type or ClC-3 overexpression by murine tail gene test. Compared with WT group, the body weight and the length of tibia in ClC-3 transgene group were decreased (P <0.05); the% Tb.Ar and Tb.Wi in cancellous bone decreased (P <0.05), Tb. Sp increased (P <0.05), while Tb.N showed no statistical significance. In cortical bone, T.Ar, Ct.Ar and% Ct.Ar decreased (P <0.05) and% Ma.Ar increased P <0.05), Ma.Ar no significant change. CONCLUSIONS: Overexpression of ClC-3 resulted in decreased cortical and cancellous bone mass and decreased bone structure, suggesting that ClC-3 may be involved in bone formation and / or bone resorption.