目的分析浙江地区淋病奈瑟菌株外膜孔蛋白PⅠ基因序列及其点突变与耐药性关系,了解本地区淋病奈瑟菌株PⅠA与PⅠB基因分布及其优势基因型。方法采用高保真PCR扩增34株淋病奈瑟菌全长PⅠ基因序列,T-A克隆后测序。根据测序结果,建立多重PCR同时检测113株淋病奈瑟菌PⅠA和PⅠB基因。分析测序菌株PⅠB基因中与耐药性密切相关的G120和A121变异情况,采用二倍琼脂稀释法确定PⅠB菌株的耐药性。结果11株PⅠA+淋病奈瑟菌均为ⅠA6血清型。23株PⅠB+淋病奈瑟菌中,6株(26．1％)为ⅠB3血清型、5株(21．7％)为ⅠB3／6血清型、2株(8．7％)为ⅠB4血清型、9株(39．1％)为ⅠB3／6-ⅠB2嵌合血清型、1株(4．3％)为ⅠB2-ⅠB4嵌合血清型。所建立的多重PCR可特异和准确地对PⅠA和PⅠB基因分型,检测灵敏度为10 ng DNA模板。113株淋病奈瑟菌中,26株(23．0％)和87株(77．0％)分别携带PⅠA和PⅠB基因。经测序的23株PⅠB+淋病奈瑟菌中,1株(4．3％)G120和A121均未突变,3株(13．0％)G120或A121突变,2株8．7％)G120突变但A121缺失,17株(73．9％)双位点突变。22株G120和／或A121突变的PⅠB+菌株均对青霉素和四环素耐药。结论所建立的多重PCR可用于淋病奈瑟菌PⅠA和PⅠB基因的分型检测。本地区流行的淋病奈瑟菌主要携带PⅠB基因。ⅠA6为PⅠA菌株优势血清型。PⅠB菌株以ⅠB3／6-ⅠB2嵌合、ⅠB3和ⅠB3／6血清型常见,但主要为耐药性G120和,或A121突变株。 Objective To analyze the PⅠ gene sequence of porcine outer membrane of Epidemic Neisseria gonorrhoeae from Zhejiang Province and its relationship with point mutations and drug resistance and to understand the distribution of PⅠA and P Ⅰ B genes and their dominant genotypes in Neisseria gonorrhoeae from this area. Methods The full-length PⅠ gene sequence of 34 strains of Neisseria gonorrhoeae was amplified by high-fidelity PCR and sequenced after T-A cloning. According to the sequencing results, 113 strains of Neisseria gonorrhoeae PⅠA and PⅠB genes were detected simultaneously by multiplex PCR. The variation of G120 and A121, which are closely related to drug resistance in PⅠB gene of sequencing strain, was analyzed. The drug resistance of PⅠB strain was determined by double agar dilution method. Results 11 strains of PⅠA + Neisseria gonorrhoeae were all ⅠA6 serotypes. Among 23 strains of PⅠB + Neisseria gonorrhoeae, 6 (26.1%) belonged to serogroup ⅠB3, 5 (21.7%) serogroup ⅠB3 / 6 and 2 strains (8.7%) serogroup ⅠB4, Nine strains (39.1%) were chimeric serotype IB3 / 6-IB2 and one strain (4.3%) was chimeric serotype IB2-IB. The established multiplex PCR can specifically and accurately genotype PⅠA and PⅠB with the detection sensitivity of 10 ng DNA template. Among 113 strains of Neisseria gonorrhoeae, 26 strains (23.0%) and 87 strains (77.0%) carried PⅠA and PⅠB genes, respectively. Of the 23 strains of PIB + gonorrhea Neisseria gonorrhoeae, 1 (4.3%) did not mutate both G120 and A121, 3 (13.0%) had G120 or A121 mutations, and 2 had 8.7%) G120 mutations A121 deletion, 17 (73.9%) double-site mutation. Twenty-two G1P and / or A121 mutated PIB + strains were resistant to penicillin and tetracycline. Conclusion The multiplex PCR can be used to detect the genotypes of PⅠA and PⅠB genes of Neisseria gonorrhoeae. Neisseria gonorrhoeae, which is endemic in this area, mainly carries PⅠB gene. ⅠA6 PⅠA dominant serotype strains. The PIB strain was chimeric with IB3 / 6-IB2, and IB3 and IB3 / 6 serotypes were common but predominantly resistant to G120 and / or A121 mutants.