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目的:评价丙氨酰-谷氨酰胺双肽(Ala-Gln)TPN对放射性肠炎大鼠结局的影响。方法:将70只大鼠随机分为四组,即对照组(n=10);实验组腹部接受单剂9.5 Gy60Co照射(AR),分为腹部辐射组(n=20);腹部辐射+TPN组(n=20);腹部辐射+TPN+Ala-Gln组(n=20)。分别观察大鼠死亡率、体质量、小肠绒毛高度和面积,肝、脾、门静脉血、肠系膜淋巴结,腹腔渗出液作细菌培养,检测血清肿瘤坏死因子-α(TNF-α)和可溶性白细胞介素2受体(sIL-2R)。结果:腹部辐射+TPN+Ala-Gln组大鼠7 d和14 d时死亡率和体质量丢失均最低;绒毛高度和面积仅轻度下降;肝、脾、门静脉血、肠系膜淋巴结和腹腔渗出液中细菌培养阳性率亦最低;血清TNF-α、sIL-2R恢复最快。结论:TPN液中添加Ala-Gln治疗放射性肠炎大鼠,可显著减少体质量丢失,减少肠道细菌移位,增加小肠绒毛高度和面积,改善机体免疫功能,降低病死率,从而显著改善放射性肠炎大鼠的预后。
Objective: To evaluate the effect of Ala-Gln TPN on the outcome of radiation-induced enteritis in rats. Methods: Seventy rats were randomly divided into four groups (control group, n = 10). The experimental group received single dose of 9.5 Gy of 60Co irradiation (AR), divided into abdominal radiation group (n = 20) Group (n = 20); Abdominal radiation + TPN + Ala-Gln group (n = 20). The mortality, body weight, villus height and area of the intestine, liver, spleen, portal vein blood, mesenteric lymph nodes and peritoneal exudate were observed for bacterial culture. Serum levels of tumor necrosis factor-α (TNF-α) and soluble interleukin 2 receptor (sIL-2R). Results: The death rate and body weight loss were the lowest on the 7th day and the 14th day in the abdominal radiation + TPN + Ala-Gln group. The height and area of villus decreased only slightly. The exudation of liver, spleen, portal vein, mesenteric lymph node and peritoneal cavity The positive rate of bacterial culture in liquid was the lowest. Serum TNF-α and sIL-2R recovered most rapidly. CONCLUSION: The addition of Ala-Gln to TPN solution to radiation-induced enteritis in rats significantly reduces body mass loss, reduces intestinal bacterial translocation, increases villus height and area in the intestine, improves immune function, reduces mortality and significantly improves radiation enteritis The prognosis of rats.